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Primary (inherited) – less common disorders in which the body is not able to produce one or more class of immunoglobulin There are a number of conditions associated with decreased and increased immunoglobulin levels. Abnormal Igs are not diagnostic but can, in association with an appropriate clinical history and other tests results, be a strong indicator of a disease or condition. Abnormal test results indicate that there is something affecting the immune system and may suggest the need for further testing. IgG, IgA, and IgM levels are usually evaluated together. Laboratory levels can vary by laboratory due to differences in testing technique. Turbidimetry measures the decrease in the intensity of light and nephelometry measures scatter of light as it passes through the sample, which is proportional to the concentration of the immunoglobulin in the solution. In both nephelometry and turbidimetry, a light source is projected through a liquid sample within a transparent container. Nephelometry and turbidimetry are the most widely used methods because of their speed, ease of use and precision. The number of infections increases if the child attends childcare, has older siblings and/or parents who smoke. In Australia, normal healthy toddlers can have up to 12 infections a year. Otherwise healthy thriving children with frequent ‘colds’ do not need their immunoglobulin levels measured. myeloma), serum immunoglobulin tests may also be ordered periodically to monitor disease progression. Measurement of IgM in a newborn may assist in the diagnosis of a congenital or neonatal infection.ĭepending on the condition (eg. 3 The tests should also be ordered along with other tests, including serum and urine electrophoresis, when there is suspicion for B-cell malignancy such as myeloma, lymphoma or chronic lymphoid leukaemia (CLL). rheumatoid arthritis, systemic lupus erythematosus, autoimmune liver disease) or chronic infections (eg. Serum immunoglobulin tests may be used in the assessment of conditions associated with chronic inflammation (eg. 2 These may occur as an isolated defect or as part of a wider combined immunodeficiency affecting both T- and B-cells. There are many types of antibody deficiencies ranging from isolated IgA deficiency to severe deficiencies of all immunoglobulins. Patients with antibody deficiency are particularly predisposed to recurrent sinopulmonary infections, especially with polysaccharide encapsulated organisms including Streptococcus pneumoniae and Haemophilus influenzae. The tests should be ordered if a patient has symptoms suggestive of an immunoglobulin deficiency, such as family history of immunodeficiency, recurrent or severe or unusual bacterial infections, lack of response to antibiotics, unusual or recurrent viral infections and/or chronic unexplained diarrhoea. A low level of immunoglobulin is termed ‘hypogammaglobulinaemia’. Serum immunoglobulin tests are used for the evaluation of antibody (humoral) immunodeficiencies. IgD and IgE will not be discussed in this article. Some conditions cause excess levels, some cause deficiencies, and others cause a combination of increased and decreased levels. Testing is used to help diagnose various conditions and diseases that affect the levels of one or more of these immunoglobulin classes. Quantitative serum immunoglobulin tests are used to detect abnormal levels of the three major classes (IgG, IgA and IgM). In normal serum, about 80% is IgG, 15% is IgA, 5% is IgM, 0.2% is IgD and a trace is IgE. There are five classes of immunoglobulin (Ig): IgG, IgM, IgA, IgD and IgE. They contain antibody activity and are produced by the terminal cells of B-cell differentiation known as ‘plasma cells’. It considers areas such as indications, what to tell the patient, what the test can and cannot tell you, and interpretation of results. 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